Molecular Characterization of bla OXA-51 Gene among Acinetobacter baumannii Isolates in Babylon Province

Authors

Huda H. Al-Hasnawy, Department of Microbiology, College of Medicine, University of Babylon, Hilla, Iraq
Alya A. Rahi, Department of Microbiology, College of Medicine, University of Babylon, Hilla, IraqFollow
Baraa H. Hadi, Department of Microbiology, College of Medicine, University of Babylon, Hilla, Iraq

Document Type

Original Study

Keywords

A.baumannii, blaOXA-51, VITEK 2 system, CHROMID

Abstract

Background: Acinetobacter baumannii, identified as an ESKAPE pathogen, is part of a group of bacteria known for their high antibiotic resistance and significant role in nosocomial infections. Objectives: The objective of the current study is to investigate the genetic sequences of the blaOXA-51 gene in A. baumannii isolates. Materials and Methods: 600 specimens were collected during the period from September 2022 to December 2022 from three hospitals in Babylon Province/ Iraq. Results: In the present study, 20 (3.33%) out of 600 isolates from (urine, Burn, blood and wound)in percentage 6(30%), 5(25%), 5(25%), and 4(20%) respectively. The identification of bacteria by VITEK 2 system and PCR techniques for the detection of bla_oxa-51gene and nucleotide sequence of nucleotide to blaoxa-51gene. The specimens were immediately inoculated on MacConkey agar and then on CHROM agar incubated overnight at 37°C under aerobic conditions, and the isolated bacteria were identified by VITEK 2 system. The detection of antimicrobial activity using the Vitek 2 system. The susceptibility of A.baumannii isolates to antibiotics, including highly resistant for Amikacin, Cefepime, Ceftazidime, and Ciprofloxacin, Piperacillin, Ticarcillin, and Ticarcillin/Clavulanic Acid. Colistin 33.33%, Imipenem 93.3%, Meropenem 86.67%, and Tobramycin 80%. Genetically, PCR for the detection of bla _OXA-51 genes among A.baumannii isolates revealed that 5 isolates were registered in GenBank. Conclusion: PCR of A. baumannii performed by blaOXA-51 Gene, Housekeeping genes blaOXA-51 are considered unique gene to A.baumannii and blaOXA-51 is considered a better molecular marker for the study of phylogenetic and taxonomic relationships at the species level, precisely for this bacterium. The detection of blaOXA-51 gene provides a correct and convenient method of identifying A. baumannii.

How to Cite This Article

Al-Hasnawy, Huda H.; Rahi, Alya A.; and Hadi, Baraa H. (2024) "Molecular Characterization of bla OXA-51 Gene among Acinetobacter baumannii Isolates in Babylon Province," Hilla University College Journal For Medical Science: Vol. 2: Iss. 2, Article 5.
DOI: https://doi.org/10.62445/2958-4515.1014