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Hilla University College Journal For Medical Science

Document Type

Original Study

Keywords

Pseudomonas aeruginosa, Biofilm formation, Antibiotic resistance

Abstract

Background: Pseudomonas aeruginosa, is a Gram-negative nosocomial pathogen implicated in various human infections, primarily associated with healthcare services. Biofilms are known for their resistance to antimicrobial agents.

Objectives: The study aims to investigate the correlation between biofilm formation and antibiotic resistance in isolates.

Materials and Methods: The samples were collected from (110) patients admitted to two main hospitals in Hilla city: Al-Hilla General Teaching Hospital, and Mergan Teaching Hospital. The specimens were collected from various clinical sites such as wounds, ears, and urine.

Results: Out of (70) positive cultures cultured on Cerimide agar medium, only 15 (13.6%) specimens showed positive identification as P. aeruginosa. Out of the total 110 specimens, 70 (63.6%) showed positive bacterial cultures. The results showed that all P. aeruginosa isolates were resistant to 12 antimicrobial agents. Except for colistin and ciprofloxacin, the results showed the highest rate of resistance against Ticarcillin, Ticarcillin /clavulanic acid, Piperacillin, Amikacin Ceftazidime, Cefepime, Imipenem, and Gentamycin. P. aeruginosa isolates 2 (13.33%) isolates had strong biofilm formation, moderate biofilm was observed in 6 (40%) isolates, and 5 (33.3%) isolates had weak or no biofilm formation 2 (13.3%).

Conclusion: The formation and persistence of biofilms can result in elevated transfer of antibiotic resistance. A relationship was observed between, Antibiotic resistant and the level of biofilm formation.

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