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Hilla University College Journal For Medical Science

Document Type

Original Study

Keywords

Candida albicans, Orthodontic appliances, PCR technique, Virulence factors

Abstract

Background: Candida albicans is commonly found in the oral cavities of individuals with denture stomatitis, especially in middle-aged and elderly adults who use dentures and orthodontic appliances. Objectives: This study aims to investigate the influence of orthodontic appliances on oral Candida albicans and demonstrate the isolation and identification of C. albicans from orthodontic patients. Materials ans Methods: Eighty swab samples were collected for culture preparation. The swabs were cultured on various culture media (such as Influence of Orthodontic Appliances on Oral Candida albicans and Molecular Study of their virulence factors, SDA, and Chrom agar) and the culture is examined for microbial growth. Microscopic examination and PCR technique were also used for identifying the isolated yeasts. Results: The current investigation showed an infection with opportunistic microorganisms as follows: 17% of C. albicans from two times (T1and T2) are positive for Candida albicans in orthodontic patients and negative in (baseline T0). In orthodontic isolates, the (ALS1) gene was strongly found in C. albicans. Conclusion: Oral Candida albicans was more prevalent in orthodontic patients. Among orthodontic patients, Candida albicans was more prevalent than other Candida species. Oral Candida albicans with the gene (ALS1) was more prevalent with biofilm formation.

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