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Hilla University College Journal For Medical Science

Document Type

Original Study

Keywords

Antibiotic resistance, Escherichia coli B2, yjaA, chuA, UTI, Phylogenetics

Abstract

Background: Urinary tract infections (UTIs) are the most prevalent bacterial illnesses. Antibiotic resistance is a common problem in E. coli bacteria, which can develop or acquire a wide range of resistance mechanisms. E. coli strains B2 carry more virulence factors that cause antibiotic resistance.

Objectives: The study aims to detect the phylogeny of antibiotic resistance genes of Escherichia coli B2 and identify the presence of the yjaA, chuA genes. It also aims to measure the susceptibility of antibiotic samples obtained from individuals diagnosed with UTI in Babylon, Iraq

Materials and Methods: The antibiotic susceptibility was measured using the disk diffusion method. These isolates were previously identified using the VITEK-2 System. The presence of the yjaA and chuA genes was then detected using specific primer pairs by polymerase chain reaction to phylogenetic E.coli B2.

Results: The total number of samples was 315, 210 (67%) positive growth and 105 (33%) showing no growth. Out of 210, 46 (22%) were E. coli. Out of 46 E. coli isolates, the percentage of B2 was 24 (52%). E.coli infection rate was 39 (85%) in females and 7 (15%) in males. Ampicillin and cephalothin showed the highest resistance at 94%, while the antibiotics Colstin and Nitrofurantoin exhibited the lowest resistance at 0% and 6%.

Conclusion: The study demonstrated that E. coli phylogenetics indicated that the most prevalent strain was B2. The antibiotics colstin, Imipenem, and Nitrofurantoin showed the lowest resistance among other antibiotics, Beta-lactam antibiotics, such as ampicillin and cephalothin exhibited the highest resistance among other antibiotics.

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